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Dick and Quinn 1995 ; Liu et al. 1991 ; Sarnaik et al. 2006 ) bacteriostatic and deadly effects may occur ( Fox et al. 2007 ). Some pesticides had been poisonous and inhibited rhizobial progress and survival ( Graham et al. 1980 ; Mallik and Tesfai 1983 ; Singh and Wright 2002 ; dos Santos et al. 2005). It’s clear that N2-fixing vegetation are extra sensitive to acidity than crops of the identical species that feed on mineral nitrogen ( Andrews 1976 ). Growth of rhizobia in acidified culture media has proved useful for choosing strains with an ability to colonize the rhizosphere and to nodulate their host plant in acid soils ( Cooper 1988 ). A serious

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